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. 2018 Nov 15;10(11):3504–3513.

Figure 2.

Figure 2

Effects of MnTBAP on Aldo induced HK-2 apoptosis and cell phenotypic alteration. A: TUNEL staining (×400). The arrows indicate TUNEL-positive apoptotic cells. B: HK-2 cell apoptosis following MnTBAP was detected by flow cytometry. Cells were pretreated with MnTBAP (100 µM) for 30 min, followed by incubation with Aldo (100 nM) for 24 h. C: Western blot of vimentin and α-SMA were performed. D: Semi-quantitative analysis of vimentin and α-SMA normalized against β-actin. Data represented as the mean ± SEM (n = 6). *P < 0.01 vs Control; #P < 0.01 versus Aldo-induced group.