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NGF stimulates capillary-like tube formation in vitro. Representative images of capillary-like tube formation of rat brain-derived micro-endothelial cells treated with vehicle (A), NGF (B), or NGF + LY294002 (C). NGF promoted capillary-like tube formation of rat cerebral rBMECs at 500 μg/ml. Bar graph showing quantification of the length of capillary-like tube formation for each group (D). *P < 0.05 versus vehicle group, and #P < 0.05 versus NGF group. Scale bar, 100 μm.
