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. 2018 Dec 6;10:399. doi: 10.3389/fnagi.2018.00399

FIGURE 5.

FIGURE 5

Hippocampal-derived MAM fractions contain RyR2, which is enriched in the MAM fraction from AβOs-injected rats. Western blot images of MAM fractions isolated from rat hippocampus (see Materials and Methods). The ER proteins analyzed were Calnexin (CNX), IP3R1, and RyR2. The inner and outer mitochondrial membrane proteins analyzed were COX1 and VDAC1, respectively; the characteristic MAM protein was ACSL-4. C, control, saline-injected; AβOs, AβOs-injected. The column at right indicates the ratios between band densities of each MAM protein (AβOs/control) normalized to b-tubulin protein levels.