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. 2018 Dec 6;9:1721. doi: 10.3389/fphys.2018.01721

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Copyright © 2018 Cabrera DeBuc, Somfai, Arthur, Kostic, Oropesa and Mendoza Santiesteban.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative image obtained with the EasyScan unit (i-Optics Corporation, Netherlands) and analyzed with the SIVA program that measured the caliber of the vessels emerging from the optic disk. Arterioles are in red and venules are in blue. The SIVA software automatically detects the optic disk and traces vessels in a zone 0.5 to 2.0-disk diameter from the disk margin. The different circular ROIs with various radii around the optic disk center are labeled as B (0.5 – 1.0 disk diameters away from the disk margin) and C (0.5- 2.0 disk diameters away from the disk).