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. 2018 Dec 6;11:447. doi: 10.3389/fnmol.2018.00447

Figure 2.

Figure 2

HIP1R is necessary for inducing neurite initiation and dendritic branching. (A) Representative images of cultured neurons electroporated with Scrambled-RNAi (Scrambled) or HIP1R-RNAi. GFP (green), MAP2 (blue) and phalloidin (red). Arrows indicate GFP-positive cells expressing Scrambled-RNAi or HIP1R-RNAi. Scale bar, 20 μm. (B) Quantification of neurite initiation (n = 3,749 neurons for the Scrambled group and 3,045 neurons for the RNAi group). (C) Representative images of day in vitro (DIV) 5–6 cultured neurons electroporated with Scrambled-RNAi or HIP1R-RNAi. MAP2 was used to outline the dendrites. Scale bar, 20 μm. (D) Cumulative distribution of primary dendritic number showed that HIP1R-RNAi neurons had fewer primary branches than Scrambled control. (E–H) Quantification of dendritic number and dendritic length in the Scrambled and HIP1R-RNAi groups (n = 106 and 151 neurons from more than three independent experiments). Mean ± SEM; **p < 0.01, ****p < 0.0001; ns, not significant; unpaired two-tailed t-test. Scr, scrambled-RNAi.