Figure 5.

HIP1R knockdown affects the internalization of EGF. (A) Representative images of control neurons after a 20 min-treatment of Alexa⁶⁴⁷-EGF stained against EGFR (red). GFP (green) and Alexa⁶⁴⁷-EGF (gray). Scale bar, 20 μm. (B) Representative images of Alexa⁶⁴⁷-EGF treated control neurons stained against EEA1 (red). GFP (green) and Alexa⁶⁴⁷-EGF (gray). Scale bar, 20 μm. (C) Representative images of Alexa⁶⁴⁷-EGF treated control and knockdown neurons. Scale bar, 20 μm. (D) Quantification of EGF clusters (n = 73 neurons for Scrambled and 51 neurons for RNAi). Mean ± SEM; ****p < 0.0001; three independent experiments; unpaired two-tailed t-test.