Figure 7.

HIP1R interacts with EGFR via amino acid 633–822 fragment. (A,B) EGFR interacts with HIP1R in cultured hippocampal neurons harvested at DIV 6–8. (A) Neuron lysates were immunoprecipitated with an anti-EGFR antibody. Lysates (input) and precipitates (Immunoprecipitation, IP: IgG and IP: EGFR) were blotted with anti-EGFR, anti-HIP1R and anti-actin antibodies. (B) Neurons electroporated with GFP-HIP1R or GFP-control were immunoprecipitated with anti-GFP antibody. (C) EGFR interacts with HIP1R in hippocampus tissue dissected from 6-week-old male mice. (D) GST-pull down of EGFR using purified GST-HIP1R_1–632, GST-HIP1R_633–822 and GST-HIP1R_823–1068 in cultured hippocampal neurons harvested at DIV 6–8.