Figure 4. Transcriptomic analysis of med14 and uvh6 mutants at 37°C.

(A) Transcriptional changes in WT (L5 background) plants subjected to heat stress (top), in med14-3 at 37°C (middle) and uvh6-3 at 37°C (bottom) relative to the WT at 37°C, represented along the chromosome five by log₂ ratios of mean reads per kilobase per million mapped reads (RPKM) values calculated in 100 kb windows. (B) Number of PCGs and TEs detected as DEGs in med14-3 at 37°C and uvh6-3 at 37°C relative to the WT at 37°C. (C) Venn diagrams showing the extent of the overlap between up-regulated and down-regulated loci determined in med14-3 at 37°C and uvh6-3 at 37°C. (D) Log₂ fold change values at 37°C versus 23°C in med14-3 (left) and uvh6-3 (right) plotted against the log₂ fold change values at 37°C versus 23°C in the WT (x axis), considering TEs up-regulated in heat-stressed WT plants.