Figure S1. Heat stress–induced release of silencing does not require ARP6 and AtMORC6.

Transcript accumulation in rosette leaves from Col-0 WT, arp6-1, and atmorc6-3 mutants was quantified by RT-qPCR at five loci overexpressed in heat stress. Samples had been subjected to a control stress at 23°C or a heat stress treatment at 37°C. Data were normalized to the geometric mean of the reference genes AT5G12240 and ACT2. Values were further normalized to the mean of WT samples at 23°C. Error bars indicate standard errors of the mean across three biological replicates. For each locus, differences in mean at 37°C between the WT and the mutants was tested using unpaired two-tailed t test, but did not reveal any significant difference (P > 0.05).