Table II.
1H and 13C chemical shifts of reference standards, glycan released from gSRR1 and glycan units present in intact gSRR1
| NMR characterization of the sSRR1 released glycans | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | CH3 | C=O | ||
| Reference Standards | |||||||||
| α-GlcNAc | H | 5.21 | 3.88 | 3.78 | 3.50 | 3.86 | 3.86,3.80 | 2.06 | – |
| C | 93.70 | 56.96 | 73.52 | 72.91 | 74.44 | 63.42 | 24.77 | 177.40 | |
| β-GlcNAc | H | 4.72 | 3.68 | 3.55 | 3.47 | 3.47 | 3.92,3.76 | 2.06 | – |
| C | 97.79 | 59.54 | 76.73 | 72.69 | 78.81 | 63.58 | 25.05 | 177.65 | |
| GlcNAc-ol (R) | H | 3.64,3.74 | 4.08 | 3.97 | 3.60 | 3.76 | 3.66,3.83 | 2.06 | – |
| C | 63.68 | 56.58 | 71.14 | 73.79 | 73.93 | 65.62 | 24.96 | 177.35 | |
| Glycan released from gSRR1, β-GlcNAc-(1→6)-GlcNAc-ol | |||||||||
| β-GlcNAc(1→(B) | H | 4.55 | 3.75 | 3.57 | 3.46 | 3.47 | 3.95,3.76 | 2.07 | – |
| C | 104.45 | 58.44 | 76.65 | 72.81 | 78.68 | 63.58 | 25.09 | 177.65 | |
| →6)GlcNAc-ol (G) | H | 3.64,3.74 | 4.08 | 3.97 | 3.60 | 3.84 | 4.09 | 2.05 | – |
| C | 63.73 | 56.55 | 70.95 | 73.65 | 72.49 | 73.75 | 24.94 | 177.35 | |
| GlcNAc units present in gSRR1, M = monosaccharide, D = disaccharide side-chain | |||||||||
| t-α-GlcNAc→Ser (αM) | H | 4.87 | 3.92 | 3.72 | 3.47 | 3.62 | 3.84,3.78 | ~2.05 | – |
| C | 100.61 | 56.35 | 73.86 | 72.68 | 75.15 | 63.44 | ~25.0 | ~177.0 | |
| →6)-α-GlcNAc→Ser (αD) | H | 4.88 | n.d. | n.d | n.d. | n.d. | 4.13,3.80 | n.d. | n.d. |
| C | 100.61 | n.d. | 73.87 | 72.54 | n.d. | 71.13 | n.d. | – | |
| t-β-GlcNAc(1→(βD) | H | 4.54 | 3.75 | 3.58 | 3.47 | 3.47 | 3.94,3.77 | ~2.07 | – |
| C | 104.51 | 58.41 | 76.54 | 72.67 | 78.74 | 63.68 | ~25.2 | ~177.3 | |
See also Supplementary data, Figure S5 and Table S3 for information on the expression of GtfA, GtfB and GtfC, and glycosylation of gSRR1 and Supplementary data, Figures S5 and S6 for information on the structural characterization of the gSRR1 released and native glycans by NMR.
n.d. = not determined.