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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Cell Calcium. 2018 Mar 14;72:70–80. doi: 10.1016/j.ceca.2018.03.002

Fig.3. Expression of STIM1 and STIM2 in isogenic CRC cell lines.

Fig.3.

A. Analysis of STIM1 and STIM2 expression in the HCT 116/HKH 2 cell line pair. i. Representative immunoblot of STIM1 in HCT 116 and HKH-2 cells. Calnexin was used a loading control. ii. Fold change in STIM1 protein levels in HKH-2 cells with respect to HCT 116 cells. iii. Fold change of STIM1 mRNA expression in HKH-2 cells with respect to HCT 116 determined by RT-qPCR. iv. Representative immunoblot of STIM2 in HCT 116 and HKH-2 cells. Calnexin was used a loading control. v. Fold change in STIM2 protein level in HKH-2 cells with respect to HCT 116 cells. vi. Fold change in STIM2 mRNA expression in HKH-2 cells with respect to HCT 116 determined by RT-qPCR. B. Analysis of STIM1 and STIM2 expression in the DLD-1/DKO-4 cell line pair. i. Representative immunoblot of STIM1 in DLD-1 and DKO-4 cells. Calnexin was used a loading control. ii. Fold change in STIM1 protein levels in DKO-4 cells with respect to DLD-1 cells. iii. Fold change in STIM1 mRNA expression in DKO-4 cells with respect to DLD-1 measured by RT-qPCR. iv. Fold change in STIM2 protein levels in DKO-4 cells with respect to DLD-1 cells. v. Fold change in STIM2 mRNA expression in DKO-4 cells with respect to DLD-1 measured by RT-qPCR. All bar graphs represent the mean ± SEM of eight experiments for immunoblotting and six experiments for RT-qPCR. *, ** and *** indicate P < 0.05, P < 0.01, and P < 0.001, respectively (one-sample Student’s t-test).