Fig. 4. STIM1 overexpression is sufficient to increase SOCE in HCT 116 cells.

i. Normalised fura-2 signal (when excited at 360 nm) in YFP and YFP-STIM1-transfected HCT 116 cells prior and subsequent to addition of MnCl₂. ii. First derivative of the normalised Mn²⁺ quench of fura-2 fluorescence. iii. Summary data showing the maximum value of the derivative of Mn²⁺ quench curve from iii. All graphs represent the mean ± SEM of data from three days of experiments, where 3 coverslips per cell type were imaged on each day. Each coverslip contained at least 30 YFP- or YFP-STIM1-positive cells. *** indicates P < 0.001 (Student’s t-test).