Fig. 1.

Phylogenetic relationships of subfamily II GH3s in plants. GH3s identified in three cotton species and established subfamily II GH3s from A .thaliana, S. lycopersicum, G. max, O. sativa, Z.mays and V. vinifera were included. Cotton GH3s identified here could be re-classified into subgroup 1–3. GH3s of other plants were unevenly distributed such that no SlGH3 and just one OsGH3 (OsGH3.11) clustered into subgroup 3. Members of VvGH3s, GmGH3s and cotton GH3s always clustered close to each other, exhibiting closer relationships. A neighbor-joining tree was built using MEGA 7.0 and visualized using online tool Evolview. The Gh_A and Gh_D in the upland cotton indicate the At- and Dt-subgenomes, respectively. GH3s belonging to one plant species are marked with the indicated leaf label decorations. Blocks of subgroup 1–3 are highlighted with light blue, pink and light green, respectively. Percentage bootstrap scores were calculated from 1000 iterations