Fig. 2.

High pressure in association with alkaline pH and the presence of Arg induce only partial unfolding of NS1-IB. Suspensions of NS1-IB were subjected at 2.4 kbar/0.4 kbar or at 1 bar. a Intrinsic (Trp) fluorescence of NS1-IB; b λ maximum of fluorescence vs concentration of GdnHCl; c λ maximum of fluorescence vs pH and d λ maximum of fluorescence vs concentration of Arg. An excitation at 290 nm was used for intrinsic fluorescence determination and the emission was measured between 300 and 400 nm. The results are representative of experiments performed three times