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. 2018 Dec 12;18:78. doi: 10.1186/s12896-018-0486-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Higher concentrations of NS1 are found in the supernatant of the suspensions subjected to HHP at alkaline pH. Concentration of NS1 in ZIKV NS1-IB supernatants submitted to 2.4 kbar / 0.4 kbar or to 1 bar and dialyzed against 50 mM TrisHCl at a pH of 8.5. a concentration of NS1 vs concentration of GdnHCl; b concentration of NS1 vs pH; c concentration of NS1 vs concentration of Arg. The results are representative of experiments performed three times