Figure 2. Mst1/2 are selectively required in CD8α⁺ DCs to orchestrate CD8⁺ T-cell homeostasis and function.

a, b, Frequencies of CD44^highCD62L^low (a) and CD44⁺IFN-γ⁺ cells (b) in splenic T cells from WT (n = 5), Mst1/2^ΔDC (n = 3), Batf3^−/− (n = 4) and Mst1/2^ΔDCBatf3^−/− (n = 4) mice. c, CFSE dilution of donor OT-I T cells in WT, Mst1/2^ΔDC, Batf3^−/−:WT or Batf3^−/−:Mst1/2^ΔDC mixed chimeras immunized with OVA. d, Frequency and number of IFN-γ⁺ cells among H-2K^b-OVA⁺ CD8⁺ T cells after immunization with OVA-loaded irradiated B2m^−/− splenocytes (n = 4 per genotype). e, Thymidine incorporation of OT-I T cells cultured with OVA protein- or OVA(257-264) peptide-pulsed CD8α⁺ or CD8α⁻ DCs (n = 8 per genotype). f, IL-2 from co-cultures in e (n = 6 per genotype for CD8α⁺ DCs, and n = 8 per genotype for CD8α⁻ DCs). Error bar indicates SEM. NS, not significant; *P < 0.05; **P < 0.01; one-way ANOVA in a, b; two-tailed unpaired Student’s t-test in d–f. Data summarize two (f, c), three (d, e) or four (a, b) independent experiments.