Table 2.
Sequence of primers and size of the amplified fragments for evaluation of target genes (cytocrome P450 2C [CYP2C19], cytocrome P450 3A [CYP3A24], aldo-keto reductase 1C4 [AKR1C4], and aldo-keto reductase 1D1 [AKR1D1]) and housekeeping genes (β-actin [ACTB], cyclophilin A [PPIA], glyceraldehyde-3-phosphate dehydrogenase [GAPDH], ATPase, and ribosomal protein L19 [RPL19])
| Gene | Sequence1 (5′–3′) | Product size, bp | GenBank2 access number | |
|---|---|---|---|---|
| CYP2C19 | F | CAAGAATCCCTGGACCTCAA | 193 | NM_001205152.1 |
| R | GCTTCAGCAGGAGCAGGAG | |||
| CYP3A24 | F | GGCTCTGTAAGAAGGATGTGG | 189 | NM_001129904.1 |
| R | CCAGTTCCAAAAGGCAGGTA | |||
| AKR1C4 | F | ACCAGCCTTGGAAAAGTCAC | 183 | NM_181027.2 |
| R | TGCGTCCTTACACTTCTCCA | |||
| AKR1D1 | F | AGGACACTCAAGGACCTCCA | 186 | NM_001192358.1 |
| R | ACTTCACCAAGCCAGCATCT | |||
| ACTB | F | ACTGGGACGACATGGAGAAG | 167 | NM_001009784.1 |
| R | GTACATGGCAGGGGTGTTG | |||
| PPIA | F | TCTGAGCACTGGAGAGAAAGG | 178 | AY251270.1 |
| R | GATGCCAGGACCTGTATGCT | |||
| GAPDH | F | GGCGTGAACCACGAGAAGTATAA | 394 | NM_001190390.1 |
| R | CCCTCCACGATGCCAAAGT | |||
| ATPase | F | TTCCTACTGCCCTGGAATG | 98 | NM_001009360.1 |
| R | CACGAAGATGAGAAGCGAGT | |||
| RPL19 | F | GAAATCGCCAATGCCAAC | 361 | AY158223.1 |
| R | GAGCCTTGTCTGCCTTCA | |||
1
F = Forward; R = Reverse.