Figure 3.

Effect of enterotoxigenic Escherichia coli (ETEC) K88 challenge on intestinal Na+/H+ exchanger 3 (NHE3) and sodium-glucose cotransporter 1 (SGLT1) expression in young piglets. The images of NHE3 (A) and SGLT1 (B) expression in the jejunum, ileum, and colon were captured under a fluorescence microscope (Carl Zeiss AG, Heidenheim, Germany). The immunostaining intensities of NHE3 (C) and SGLT1 (D) were quantified by ImageJ software (version 1.50; National Institutes of Health, Bethesda, MD). Piglets in the control group were fed the basal diet and challenged with sterile PBS. Piglets in the ETEC K88 group were fed the basal diet and challenged with ETEC K88. The middle portions of the jejunum, ileum, and colon were collected for immunohistochemistry. Original magnification 100x. Scale bar = 100 μm. Results are mean ± SEM (n = 6). a,bMeans with different superscripts between columns differ (P < 0.05).