Table 2.
Sequence, and product size of primers for targeted genes
| Item1 | Primer sequence2 | Product size, bp | Accession no. |
|---|---|---|---|
| AQP1 | F: TGACCTTCGCTATGTGCTTCC | 221 | NM_214454.1 |
| R: GTCCAAGTGTCCAGAGGGGTAG | |||
| AQP3 | F: TGACCTTCGCTATGTGCTTCC | 212 | NM_001110172.1 |
| R: GTCCAAGTGTCCAGAGGGGTAG | |||
| AQP7 | F: CCCGTGCCTCCAAGATGA | 58 | NM_001113438.1 |
| R: CGCATTATTGTTTGCATCTTTGA | |||
| AQP8 | F: GGTGCCATCAACAAGAAGACG | 227 | NM_001112683.1 |
| R: CCGATAAAGAACCTGATGAGCC | |||
| AQP9 | F: TGTCATTGGCCTCCTGATTG | 62 | NM_001112684.1 |
| R: TGGCACAGCCACTGTTCATC | |||
| AQP11 | F: CGTCTTGGAGTTTCTGGCTACC | 229 | NM_001112682.1 |
| R: CCTGTCCCTGACGTGATACTTG | |||
| NHE3 | F: AGCTGGAGATCATAGACCAGGTG | 147 | AF_123280 |
| R: CGGTGAAGAAGATGACGATGAG | |||
| SGLT1 | F: CCCAAATCAGAGCATTCCATTCA | 153 | NM_001164021.1 |
| R: AAGTATGGTGTGGTGGCCGGTT | |||
| NKCC1 | F: CCAATGCTGTTGCAGTTGCT | 264 | XM_005661615.2 |
| R: TGGGCTTCTTGCTCTCCAAG | |||
| CFTR | F: ACTATGGACCCTTCGAGCCT | 123 | NM_001104950.1 |
| R: CGCATTTGGAACCAGCGTAG | |||
| β-actin | F: TCCACCGCAAATGCTTCTAG | 208 | AY550069 |
| R: TGCTGTCACCTTCACCGTTC |
1
AQP = aquaporin; NHE3 = Na+/H+ exchanger 3; SGLT1 = sodium-glucose cotransporter 1; NKCC1 = Na-K-Cl cotransporter; CFTR = cystic fibrosis transmembrane conductance regulator.
2
F = forward; R = reverse.