Table 3.
Primary antibodies and dilutions used in this study
| Antibody1 | Supplier | Dilution2 |
|---|---|---|
| Rabbit polyclonal to AQP3 | Bioss Antibodies3 | WB, 1:500; IF, 1:200; and IHC, 1:500 |
| Rabbit polyclonal to AQP9 | AVIVA Systems Biology Corp.4 | WB, 1:1,000 and IF, 1:50 |
| Rabbit polyclonal to AQP11 | FabGennix International Inc.5 | WB, 1:500; IF, 1:50; and IHC, 1:500 |
| Mouse monoclonal to NHE3 | Abcam6 | WB, 1:500 and IHC, 1:500 |
| Mouse monoclonal to CFTR | Abcam | IHC, 1:300 |
| Rabbit polyclonal to SGLT1 | Abcam | IHC, 1:500 |
| Rabbit polyclonal to NKCC1 | Cell Signaling Technology, Inc7 | IHC, 1:200 |
| Rabbit polyclonal to PKA (C-α) | Cell Signaling Technology, Inc. | WB, 1:1,000 |
| Rabbit polyclonal to p-PKA (C-α; Thr197) | Cell Signaling Technology, Inc. | WB, 1:1,000 |
| Rabbit polyclonal to PKA (α/β/γ) | Santa Cruz Biotechnology8 | WB, 1:1,000 |
| Rabbit polyclonal to p-PKA (α/β/γ; Thr198) | Santa Cruz Biotechnology | WB, 1:1,000 |
| Rabbit monoclonal to CREB | Cell Signaling Technology, Inc. | WB, 1:1,000 |
| Rabbit monoclonal to p-CREB (Ser133) | Cell Signaling Technology, Inc. | WB, 1:1,000 |
| Mouse monoclonal to β-actin | Abcam | WB, 1:4,000 |
1
AQP = aquaporin; NHE3 = Na+/H+ exchanger 3; CFTR = cystic fibrosis transmembrane conductance regulator; SGLT1 = sodium-glucose cotransporter 1; NKCC1 = Na-K-Cl cotransporter; PKA = protein kinase A; p-PKA = phosphorylated PKA; CREB = cAMP-response element binding protein; p-CREB = phosphorylated CREB.
2
IF = immunofluorescence; IHC = immunohistochemistry; WB = western blotting.
3
Beijing, P. R. China.
4
San Diego, CA.
5
Frisco, TX.
6
Cambridge, MA.
7
Beverly, MA.
8
Dallas, TX.