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. 2018 Dec 10;11:8915–8923. doi: 10.2147/OTT.S185244

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© 2018 Zhuang and Lu. This work is published and licensed by Dove Medical Press Limited

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Knockdown of FAM46C regulated cell apoptosis and proliferation via ERK pathway.

Notes: HSC4 cells transduced with FAM46C shRNAs (siFAM46C) lentivirus or control shRNA (siNC) lentivirus and at same time treated with U0126 or vehicle (DMSO). (A) Cell apoptosis was evaluated by flow cytometry after 48 hours of treatment. (B) at 0, 24, 48, and 72 hours after treatment, cell proliferation of these two cell groups was evaluated by ccK-8 assay. Data are based on three independent experiments and shown as mean ± SD. (C) The protein expression levels of cleaved caspase 9, cleaved caspase 3, p-ERK1/2, and ERK1/2 were analyzed by Western blotting. ^###P<0.001 vs siNC+DMSO; ***P<0.001 vs siFAM46C+DMSO.

Abbreviation: CCK-8, Cell Counting Kit-8.