Figure 1.

Hypoxia could activate ADAR1 expression and consequently affects cell proliferation and tube formation.

Notes: (A) Relative mRNA expression level of ADAR1 genes in a time-course condition of hypoxia culture. GAPDH was used as housekeeping gene for normalization. (B) Western blot analysis of ADAR1, FGFR2, VEGF, and HIF1 proteins compared between normal and hypoxia condition. Quantitative results were normalized to GAPDH. (C) Western blot analysis of knocking down efficiency of ADAR1 shRNAs. ADAR1 protein was quantified by normalizing to GAPDH. (D) Relative mRNA expression level of FGFR2, HIF1, and VEGF genes compared in control and shADAR1 HUVECs. GAPDH was used as housekeeping gene for normalization. Values are expressed as mean ± SE. *P<0.05, as compared to the control.

Abbreviations: ADAR1, adenosine deaminase acting on RNA 1; FGFR2, fibroblast growth factor receptor 2; HIF1, hypoxia inducible factor 1; HUVEC, human umbilical vein endothelial cell; NC, negative control; VEGF, vascular endothelial growth factor.