| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse GITR (clone DTA-1) | Fc variants generated in-house | RRID: AB_1107688 |
| Anti-mouse CTLA-4 (clone 9D9) | Patent literature, Fc variants generated in-house | RRID: AB_10949609 |
| Anti-mouse/human TIGIT (clone 10A7) | Patent literature, Fc variants generated in-house | US patent number: 9,499,596 |
| Anti-mouse CD45RB (clone MB23G2) | MB23G2 hybridoma, Fc variants generated in-house | RRID: AB_1107653 |
| Anti-human CTLA-4 (internal clone) | Clone and Fc variants generated in-house | Unpublished patent application |
| Anti-human GITR (clone 6C8, Tolerx) | Patent literature | US patent number: 7,812,135 |
| Anti-mouse FoxP3 (clone FJK-16s) | Thermo Fisher | RRID: AB_469916 |
| Anti-human FOXP3 (clone PCH101) | eBioscience | RRID: AB_1834365 |
| Anti-mouse CD25 (clone PC-61.5.3) | BioXcell | RRID: AB_1107619 |
| Anti-mouse CD25 (clone 3C7) | Biolegend | RRID: AB_2616761 |
| Anti-human CD25 (clone M-A251) | Biolegend | RRID: AB_2561975 |
| Anti-mouse CD45.2 (clone 104) | Biolegend | RRID: AB_313444 |
| Anti-mouse CD4 (clone RM4-5) | Biolegend | RRID: AB_11126142 |
| Anti-mouse CD8 (clone 53-6.7) | Biolegend | RRID: AB_493426 |
| Anti-mouse Vβ2 (clone B20.6) | Biolegend | RRID: AB_1089254 |
| Anti-mouse Vβ8.1, 8.2 (clone KJ16-133.18) | Biolegend | RRID: AB_1186101 |
| Anti-mouse IL-2 (clone JES6-5H4) | Thermo Fisher | RRID: AB_315303 |
| Anti-human IL-2 (clone MQ1-17H12) | Biolegend | RRID: AB_315097 |
| Anti-mouse/human Ki67 (clone B56) | BD Biosciences | RRID: AB_396302 |
| Anti-mouse CD62L (clone MEL-14) | Biolegend | RRID: AB_2563058 |
| Anti-mouse CD44 (clone IM7) | Biolegend | RRID: AB_2564214 |
| Anti-human CD56 (clone HCD56) | Biolegend | RRID: AB_11218798 |
| Anti-human NKp46 (clone 9E2) | Miltenyi Biotec | RRID: AB_2660316 |
| Anti-human CD14 (clone 63D3) | Biolegend | RRID: AB_2687384 |
| Anti-human CD3 (clone OKT3) | Biolegend | RRID: AB_2563352 |
| Anti-human CD3 (clone SP34) | BD Biosciences | RRID: AB_396483 |
| Anti-human CD19 (clone HIB19) | Biolegend | RRID: AB_2562097 |
| Anti-human MHC-II (clone L243) | Biolegend | RRID: AB_2616625 |
| Anti-human IFNγ (clone 4S.B3) | Biolegend | RRID: AB_315237 |
| Anti-human CD11c (clone 3.9) | Biolegend | RRID: AB_389351 |
| Anti-human CD1c (clone L161) | Biolegend | RRID: AB_2629759 |
| Anti-human CD141 (clone M80) | Biolegend | RRID: AB_2572198 |
| Anti-human CD304 (clone 12C2) | Biolegend | RRID: AB_2563872 |
| Anti-human CD4 (clone OKT4) | Biolegend | RRID: AB_11204077 |
| Anti-human CD80 (clone 2D10) | Biolegend | RRID: AB_2076147 |
| Anti-human CD86 (clone IT2.2) | Biolegend | RRID: AB_10899582 |
| Anti-human CD16 (clone 3G8) | Biolegend | RRID: AB_2572006 |
| Anti-mouse CD16.2 (clone 9E9) | Biolegend | RRID: AB_2565302 |
| Anti-human CD32A (clone IV.3) | STEMCELL Technologies | RRID: AB_519584 |
| Anti-human CD32B (clone 2B6), hIgG1-N297A and mIgG2a-N297A variants | Patent literature, Fc variants generated in-house | Patent:US8968730B2 |
| Anti-human CD32A/B (clone 6C4) | Thermo Fisher | RRID: AB_1311188 |
| Anti-human CD64 (clone 10.1) | BD Biosciences | RRID: AB_627153 |
| Anti-human ZAP70 (clone 99F2) | Cell Signaling Technology | RRID: AB_10691455 |
| Anti-human pZAP70 (2704) | Cell Signaling Technology | RRID: AB_2217457 |
| Biological Samples | ||
| Healthy human peripheral blood (unpurified buffy coats and leukopheresis) | Research Blood Components, LLC: http://researchbloodcomponents.com/products.html |
N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Staphylococcal enterotoxin A (SEA) | Toxin Technology, Inc | Cat# AT101red |
| Staphylococcal enterotoxin B (SEB) | Toxin Technology, Inc | Cat# BT202red |
| Recombinant human CTLA-4 (polyhistidine tag) | Sino Biological | Cat# 11159-H08H |
| Recombinant human CTLA-4 (Fc-fusion) | R&D Systems | Cat# 7268-CT |
| Recombinant human CD80 (Fc-fusion) | R&D Systems | Cat# 140-B1 |
| Recombinant human CD86 (Fc-fusion) | R&D Systems | Cat# 141-B2 |
| Diphtheria toxin | Sigma Aldrich | D0564; MDL: MFCD00163490 |
| Critical Commercial Assays | ||
| CTLA-4 Blockade Bioassay | Promega | Cat# JA3001 |
| FOXP3 staining buffer set | eBioscience | Cat# 00-5523-00 |
| Zombie NIR fixable viability kit | Biolegend | Cat# 423106 |
| Experimental Models: Cell Lines | ||
| CT26 colon carcinoma | ATCC | RRID: CVCL_7256 |
| CHO-mouse-FcγRI | CNCM, Institut Pasteur | Described: Mancardi et al., 2008 |
| CHO-mouse-FcγRIIB | CNCM, Institut Pasteur | Described: Mancardi et al., 2008 |
| CHO-mouse-FcγRIII | CNCM, Institut Pasteur | Described: Mancardi et al., 2008 |
| CHO-mouse-FcγRIV | CNCM, Institut Pasteur | Described: Mancardi et al., 2008 |
| CHO-human-FcγRI | CNCM, Institut Pasteur | CNCM I-4383 |
| CHO-human-FcγRIIA-H/H131 | CNCM, Institut Pasteur | CNCM I-4384 |
| CHO-human-FcγRIIA-R/R131 | CNCM, Institut Pasteur | CNCM I-4385 |
| CHO-human-FcγRIIB | CNCM, Institut Pasteur | CNCM I-4386 |
| CHO-human-FcγRIIIA-F/F131 | CNCM, Institut Pasteur | CNCM I-4388 |
| CHO-human-FcγRIIIA-V/V131 | CNCM, Institut Pasteur | CNCM I-4389 |
| Jurkat-FcγRIIA-H/H131 | Promega | Cat# G9991 |
| Jurkat-CTLA-4 (forced extracellular expression) | This paper | Described: Nakaseko et al., 1999 JEM |
| Experimental Models: Organisms/Strains | ||
| Mouse: C57BL/6 | The Jackson Laboratory | Stock# 000664 |
| Mouse: BALB/cJ | The Jackson Laboratory | Stock# 000651 |
| Mouse: B6.129-FoxP3tm3(DTR/GFP)Ayr/J | The Jackson Laboratory | Stock# 016958 |
| Software and Algorithms | ||
| WEHI Weasel software v3.2.1 | WEHI Institute | Information |
| Prism 7 | Graphpad | Information |
| FACSDIVA | BD Biosciences | Information |
| ImageJ | Wayne Rasband, NIH | Information |
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