Figure 1. Schematic representation of evaluation of neuraminidase inhibitors using DIANA (A) and preparation of a detection probe (B).

(A) The detection probe binds to the active site of neuraminidase captured on the well by an immobilized anti-NA antibody. The amount of bound detection probe is determined by qPCR. The inhibition potency of the tested compound can be determined from the difference in the amount of bound probe after incubation of neuraminidase in the presence and absence of the compound. (B) Preparation of a covalent conjugate consisting of a reporter DNA oligonucleotide and a neuraminidase inhibitor (compound 1) used as a DIANA detection probe. Only one regioisomer of SPAAC product is shown.