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. 2018 Dec 10;475(23):3827–3846. doi: 10.1042/BCJ20180347

Figure 3. TIPARP interacts with specific sequences in its N-terminus and catalytic domains.

Figure 3.

(A) TIPARP interacts with sequences in its N-terminal region and its catalytic domain. 20-mer peptides with four amino acid offset covering the entire sequence of TIPARP were spotted on a membrane that was incubated with GST or GST-TIPARP that were detected by immunoblotting for GST. (B) The peptide sequences that specifically bound GST-TIPARP are shown in bold font. (C) Schematic showing the relative position of the interacting peptide sequences to known functional in TIPARP. (D) COS1 cells were transfected with FLAG-TIPARP1-657 and GFP-TIPARP (full-length; 1–657) or GFP-TIPARP 33–657. Cell extracts were prepared 24 h later and immunoprecipitated with anti-FLAG followed by western blotting with the indicated antibodies. The data are representative of three independent experiments.