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. 2018 Dec 10;475(23):3827–3846. doi: 10.1042/BCJ20180347

Figure 7. Increased TIPARP expression increases mono-ADP-ribosylation of AHR and represses AHR signalling.

Figure 7.

MCF7 cells expressing a Tet-ON regulated HA-tagged TIPARP were cultured in the presence or absence of dox and treated with DMSO or 10 nM of TCDD for 20 h. The mRNA expression levels of (A) TIPARP and (B) CYP1A1 were determined as described in the Materials and Methods. (C) The increased mono-ADP-ribosylation of AHR in the presence of increased expression of TIPARP. Mono-ADP-ribosylation of immunoprecipitated AHR by TIPARP was detected by anti-MAR reagent. The data are representative of three independent experiments. *P < 0.05 compared with no dox DMSO-treated cells. #P < 0.05 compared with TIPARP-transfected cells treated with TCDD.