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. Author manuscript; available in PMC: 2019 Jun 3.
Published in final edited form as: Nat Struct Mol Biol. 2018 Dec 3;25(12):1119–1127. doi: 10.1038/s41594-018-0156-z

Figure 1. TFIID and SAGA submodules in the cytoplasm.

Figure 1

a, Cellular localization of GFP-TAF5, GFP-TAF9 and GFP-TAF6 by confocal microscopy; scale bar = 10 μm. b, GFP-TAF5 and GFP-TAF6 enrich all TFIID subunits in co-IPs from nuclear extracts; GFP-TAF9 enriches TFIID and SAGA subunits. Subunits unique to TFIID are colored in green, subunits unique to SAGA in purple, shared subunits in blue. Dashed red lines denote threshold between background and significant enrichment (two-tailed t-test; FDR = 1%; S0 =1). Each data point is plotted as average of technical triplicates. c, Relative abundance of TFIID and SAGA subunits from nuclear extracts are shown, normalized to TAF4/4B. Each bar represents an average of technical triplicates. Error bars = s.d. of mean. d, GFP-TAF5 and GFP-TAF6 enrich a subset of TFIID subunits in co-IPs from the cytoplasm; GFP-TAF9 enriches both TFIID and SAGA subunits. Each data point is plotted as average of technical triplicates. e, Relative abundance of TFIID and SAGA subunits in the cytoplasmic co-IPs. Each bar represents an average of technical triplicates. Error bars = s.d. of mean. Source data for c and e are available online.