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. Author manuscript; available in PMC: 2019 Jun 3.
Published in final edited form as: Nat Struct Mol Biol. 2018 Dec 3;25(12):1119–1127. doi: 10.1038/s41594-018-0156-z

Figure 6. CCT chaperonin-assisted early events in TFIID assembly.

Figure 6

a, siRNA-mediated knockdown of CCT subunit TCP1 impedes GFP-TAF5 integration into TFIID. Control cells were treated with a non-targeting siRNA (siNT). Input represent 2% of the protein sample used in each co-IP. Technical replicates n = 2. Uncropped blot/gel images are shown in Supplementary Data Set 1. b, TCP1 knockdown affects nuclear localization of GFP-TAF5; scale bar = 10 μm. Technical replicates n = 2. c, Newly- synthesized TAF5 is captured by the chaperonin (left). The TAF5 N-terminal domain folds autonomously, Folding of the WD40 repeat domain depends on CCT. Release of readily folded TAF5 from the chaperonin requires binding of TAF6-TAF9, resulting in a discrete TAF5-TAF6-TAF9 complex in the cytoplasm. Binding of further TAFs and TBP completes functional holo-TFIID assembly.