Real-time qPCR of viral DNA in wild-type HSV-1 control and αHT-treated samples, performed in triplicate. The decrease in HSV-1 DNA copy number is significant, as determined by one-way ANOVA and Tukey’s post-hoc analysis (**, p < 0.05). No significant differences were observed between αHT treated samples (50 μM αHT-106, 30 μM αHT-111, or 8 μM αHT-115).