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. Author manuscript; available in PMC: 2018 Dec 13.
Published in final edited form as: Methods Mol Biol. 2015;1271:267–292. doi: 10.1007/978-1-4939-2330-4_18

Fig. 3.

Fig. 3

Single micrograph of a ROS taken during a tilt series. (a) Image corresponds to the 0° tilt angle. (b) Projection (6 nm thick) from a tomogram (same cell as in a) of the inner segment, containing the base of the connecting cilium and attached structures. This projection reveals the contrast gained from tomographic reconstruction of the tilt series micrographs. Scale bars = 300 nm