Fig 5. CFT073^HC mice are protected against CFT073 rUTI, but not against UTI89 rUTI.

Mice were initially infected with 10⁸ CFU UTI89 or CFT073 and monitored over four weeks, at which time antibiotic therapy was initiated. Those mice that developed chronic cystitis during the initial infection were then challenged four weeks after antibiotics (A-C) or six months after antibiotics (D) with 10⁷ CFU UTI89 or CFT073 as indicated. Initially mock-infected mice were included as a control (“Adult Naive”). (A) Mice were sacrificed six hours after infection with CFT073 and bladder and kidney titers were assessed. * P < 0.05, Kruskal-Wallis test with Dunn’s multiple test correction. (B and C). Mice were challenged with UTI89 or CFT073 and followed over four weeks. (B) Urine titers at 24 hours post challenge, representing acute cystitis. (C) Incidence of chronic cystitis over the four week challenge. * P < 0.05, Fisher’s exact test. (D) Incidence of chronic cystitis over the four week challenge, six months after the initiation of antibiotics, compared to age-matched (“Naive”) mice and mice that had resolved their initial infection (“UTI89^HR”). * P < 0.05, Fisher’s exact test. # denotes the number of mice per group. Data are from two to three independent experiments. Data points represent actual values for each individual mouse, zeros are plotted at the limit of detection, and bars indicate median values.