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. 2018 Dec 13;14(12):e1007193. doi: 10.1371/journal.ppat.1007193

Fig 4. Generation of F1-generation TG pigs.

Fig 4

(A) Photographs show one of the F1-generation TG piglets. (B) The knock-in events in newborn TG piglets were confirmed by genomic PCR. Pigs 0042, 0047, 0049, 0050, 0058 and 0059 are TG pigs, and pigs 0041, 0044, 0045, 0060 and 0061 were NTG. M, DNA marker (D2000, Tiangen, Beijing, China). (C) The relative expression levels of the targeting siRNA in F0- and F1-transgenic cells were compared by qPCR. Targeting siRNA expression levels were analysed with the unpaired t-test (ns. not significant). Error bars represent the SEMs, n = 3. TG-F1 represents F0-generation TG pigs. TG-F1 represents F1-generation TG pigs. (D) qPCR results show the relative expression of viral RNA in NTG cells, F0-generation TG cells and F1-generation TG cells. Viral RNA expression was analysed with an unpaired t-test (ns. not significant; ***p<0.001). Error bars represent the SEMs, n = 3.