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. 2018 Dec 13;13(12):e0208936. doi: 10.1371/journal.pone.0208936

Fig 3. Human endogenous retrovirus expression in TRIM28 CRISPR clones.

Fig 3

The expression of four human endogenous retroviruses up-regulated in TRIM28-mutant WT was evaluated in HEK293 cells (HEK293 parent), two CRISPR clones with normal TRIM28 gene and protein levels (CRISPR Ctrl 1 and 2), and three TRIM28 knockdown CRISPR clones (CRISPR Clone 1, 2, and 3) by SYBR Green PCR. The endogenous retrovirus levels were normalized to GAPDH and are presented as the relative quantitative (RQ) value compared to HEK293 parent cells. Error bars represent the standard deviation of two PCR replicates.