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. 2018 Dec 3;12(12):e0007014. doi: 10.1371/journal.pntd.0007014

Fig 2. The specificity of HSS-Ab and NSS-Ab.

Fig 2

(A, B) HSS-Ab (A) and NSS-Ab (B) were serially diluted from 1:2000 to 1:16000 and used as primary antibodies to detect four snake venoms. The assay was performed in duplicate, and results are presented as average values in relative fluorescence units (RFUs). (C, D) Venom proteins (5μg) from T. stejnegeri (TA), P. mucrosquamatus (PM), B. multicinctus (BM), and N. atra (NA) were resolved by SDS-PAGE on 15% gels, transferred to PVDF membranes, and probed with HSS-Ab (C) and NSS-Ab (D) (2 μg/ml) as primary antibodies.