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Each mouse was injected with the MLD of venom proteins from (A) T. stejnegeri, (B) P. mucrosquamatus, (C) B. multicinctus or (D) N. atra, and plasma samples were collected from venous blood at four time-points (30 min, 1 h, 1.5 h and 2 h) post injection. Venom concentrations were determined by analyzing the collected plasma samples using HSS-Ab–based (A and B) or NSS-Ab–based (C and D) sandwich ELISAs.
