Key resources table.
| Reagent type | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, or strain background (Mus musculus, C57BL/6J) |
Atxn1154Q/2Q KI | PMID:12086639 | RRID:MGI:2429435, backcrossed in house on C57BL/6J background | |
| Strain, or strain background (Mus musculus, FVB/NJ) |
ATXN1-82Q Tg | PMID:7553854 | RRID:MGI:2447854
backcrossed in house on FVB/NJ background |
|
| Strain, or strain background (Mus musculus, C57BL/6J) |
C57BL/6J | The Jackson Lab | Stock: 000664 | |
| Strain, or strain background (Mus musculus, FVB/NJ) |
FVB/NJ | The Jackson Lab | Stock: 001800 | |
| Cell line (Mus musculus) |
BV2 | A kind gift from Dr. Katerina Akassoglou (Adams et al., 2007) | RRID: CVCL_0182 | |
| Transfected construct |
human Flag-ATXN1 82Q | A kind gift from Dr. Huda Zoghbi | Addgene Plasmid #33237 | |
| Transfected construct |
mammalian expression vector pcDNA3.1+ | Invitrogen (ThermoFisher Scientific) | Cat. # V790-20 | |
| antibody | rabbit polyclonal anti-Atxn111750 | a kind gift from Huda Zoghbi (Servadio et al., 1995) | RRID:AB_2721278 | (1:1,000) WB |
| Antibody | mouse monoclonal anti-Calbindin | Sigma | Cat. #: C9848 RRID:AB_476894 |
(1:2,000) IHC |
| Antibody | mouse monoclonal anti-Gapdh | Sigma | Cat. #G8795 RRID:AB_1078991 |
(1:10,000) WB |
| Antibody | chicken polyclonal anti-Gfap | Abcam | Cat. # ab4674 RRID:AB_304558 |
(1:1,000) IHC |
| Antibody | rabbit polyclonal anti-Iba1 | Wako | Cat. # 019–19741 RRID:AB_839504 |
(1:2,000) IHC |
| Antibody | goat polyclonal anti-chicken Alexa555 | ThermoFisher | Cat. #A21437 RRID:AB_2535858 |
(1:500) IHC |
| Antibody | goat polyclonal anti-mouse Alexa488 | ThermoFisher | Cat. # A11001 RRID:AB_2534069 |
(1:500) IHC |
| Antibody | goat polyclonal anti-rabbit Alexa568 | ThermoFisher | Cat. #A11011 RRID:AB_143157 |
(1:500) IHC |
| Antibody | donkey anti-rabbit | GE Healthcare | Cat. #NA934 RRID:AB_772206 |
(1:4,000) WB |
| Antibody | anti-mouse | GE Healthcare | Cat. #NXA931 RRID:AB_772209 |
(1:4,000) WB |
| Commercial assay or kit | RNeasy Mini Kit | Qiagen | Cat #74106 | |
| Commercial assay or kit | iScript cDNA Synthesis Kit | Bio-Rad | Cat. #1708891 | |
| Commercial assay or kit | iTaq Universal Probes Supermix | Bio-Rad | Cat. #1725131 | |
| Commercial assay or kit | Pierce BCA Protein Assay | ThermoFisher | Cat. #23225 | |
| Commercial assay or kit | Amaxa Cell Line Nucleofector Kit T | Lonza | Cat. #VCA-1002 | |
| Other | DAPI | Vector Laboratories | Cat. #H-1500 RRID:AB_2336788 |
|
| Other | Actb probe | Applied Biosystems | Cat. #4352932E | |
| Other | ATXN1 probe | Applied Biosystems | Cat. # 4331182 Hs00165656_m1 |
|
| Other | Calb1 probe | Applied Biosystems | Cat. #4331182 Mm00486647_m1 |
|
| Other | Gapdh probe | Applied Biosystems | Cat. #4352933E | |
| Other | Ifitm3 probe | Applied Biosystems | Cat. # 4331182 Mm00847057_s1 |
|
| Other | Ifr7 probe | Applied Biosystems | Cat. #4331182 Mm00516793_g1 |
|
| Other | Oasl2 probe | Applied Biosystems | Cat. # 4331182 Mm01201449_m1 |
|
| Other | Stat1 probe | Applied Biosystems | Cat. # 4331182 Mm01257286_m1 |
|
| Software, algorithm |
Ingenuity Pathway Analysis (IPA) (Spring Release 2018) | http://www.ingenuity.com/products/pathways_analysis.html | RRID:SCR_008653 | |
| Software, algorithm |
NIH DAVID v6.8 | http://david.abcc.ncifcrf.gov/ | RRID:SCR_001881 | |
| Software, algorithm | TopHat v2.1.0 | http://tophat.cbcb.umd.edu/ | RRID:SCR_013035 | |
| Software, algorithm | Cufflinks v2.2.1 | http://cole-trapnell-lab.github.io/cufflinks/ | RRID:SCR_014597 | |
| Software, algorithm | Bowtie2 v2.1.0 | http://bowtie-bio.sourceforge.net/bowtie2/index.shtml | RRID:SCR_016368 | |
| Software, algorithm | FastQC v0.11.3 | http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ | RRID:SCR_014583 | |
| Software, algorithm | Cummerbund | http://compbio.mit.edu/cummeRbund/index.html | RRID:SCR_014568 | |
| Software, algorithm | Bioconductor R with heatmap.2 gplots package | https://www.rdocumentation.org/packages/gplots/versions/3.0.1/topics/heatmap.2 | ||
| Software, algorithm | R project for statistical computing v3.3.3 | http://www.r-project.org/ | RRID:SCR_001905 | |
| Software, algorithm | MEME Suite - Motif-based sequence analysis tools | http://meme-suite.org/ | RRID:SCR_001783 | |
| Software, algorithm | EnrichmentMap | http://baderlab.org/Software/EnrichmentMap | RRID:SCR_016052 | |
| Software, algorithm | Cytoscape v3.5.1 | http://cytoscape.org | RRID:SCR_003032 | |
| Software, algorithm |
GraphPad Prism | http://www.graphpad.com/ | RRID:SCR_002798 | |
| Software, algorithm | ImageJ | https://imagej.net/ | RRID:SCR_003070 |