Figure 3.
Evolved clones contained mutations in actP. (A) Pie chart showing types of actP mutation in single‐species and cocultured populations. All but one single‐species population contained the actP mutation, whereas it was found in none of the cocultured populations. (B) Chart indicating location of mutations in the amino acid sequence of 28 evolved clones. Mutations were not evenly distributed across ActP and tended to occur by expansion or contraction of a (TGG)4 trinucleotide repeat ∼160 codons from the start codon, indicated with a vertical line. Note that two clones were sequenced from five populations, separated by a horizontal dotted line. Genome sequencing data were supplemented by targeted sequencing of actP for single‐species plasmid‐free replicates b, e, and f, and cocultured plasmid‐free replicates b, c, e, and f.