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. Author manuscript; available in PMC: 2019 Dec 12.
Published in final edited form as: Cell Host Microbe. 2018 Nov 15;24(6):804–816.e6. doi: 10.1016/j.chom.2018.10.015

Figure 5-.

Figure 5-

Functional analysis of the multi-domain structure of TgGC (A) Microneme secretion of extracellular RH GC-mAID-3HA complement lines. Parasites were stimulated ± serum albumin/ethanol following 14 h treatment with IAA or vehicle. Western blot of T. gondii excreted/secreted antigens probed for secreted micronemes (anti-MIC2) and constitutive dense granule secretion (anti-GRA7). Stim = stimulated, mock = mock stimulation with vehicle. Representative blot shown (N = 2 similar experiments). (B) Plaques formed by RH GC-mAID-3HA complement lines on HFF monolayers with IAA or vehicle at D8, 200 parasites/ monolayer. Plaque data represented as mean ± SD (n = 9 replicates combined from N = 3 trials). Each parasite line was analyzed individually for statistical significance using an unpaired t test (IAA vs control), P values: **** ≤ 0.0001.