Figure 1. Study design and timeline.

Schematic representation of the experimental design. C57/B6 male mice were exposed to adolescent stress (F0) and then used for mating to generate the offspring. Both male and female offspring were tested for affective behaviors, and only males were used tissue collection for RNA-sequencing and mating to generate the grandoffspring (a). Diagram representation of the mice used for behavioral testing, mating, or RNA-sequencing in the stress-exposed mice, offspring, and grandoffspring (b). All mice were used only for one behavioral test, and mice used for mating or for RNA-sequencing were not evaluated in behavioral tests. Figure 1a describes the exact number of mice that were used in each behavioral test.