Figure 6.

CsH Degrades IFITM3

(A) IFITM3 protein levels were evaluated in human bulk CB-CD34⁺ cells ± IFNα by immunofluorescence and quantified as integrated density with ImageJ software after an overnight exposure to CsH (mean ± SEM of three independent experiments in duplicate, n = 18 images; Mann-Whitney test, ^∗∗∗p ≤ 0.001, ^∗∗∗∗p ≤ 0.0001) or by western blot as above (one representative blot out of two is shown).

(B) Freshly isolated FACS-sorted CD34⁺38⁻133⁺ HSC were transduced ± CsH and IFITM3 protein levels were evaluated by immunofluorescence as above (mean ± SEM, n = 10 images; Mann-Whitney test versus DMSO, ^∗p ≤ 0.05).

(C) IFITM3 protein levels, evaluated by immunofluorescence or by WB as above, in THP-1 deleted for endogenous IFITM3 overexpressing the WT or the Y20F form of IFITM3 were then exposed ± CsH (mean ± SEM of three independent experiments, n = 14 images; Mann-Whitney test, ^∗∗p ≤ 0.01).

See also Figure S6.