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. 2018 Dec 7;9:629. doi: 10.3389/fgene.2018.00629

FIGURE 3.

FIGURE 3

DLEU1 acts as a ceRNA by sponging miR-381. (A) The predicted binding site of miR-381 to the DLEU1 sequence was shown. (B) qRT-PCR analysis of miR-381 in SiHa cells transfected with DLEU1 siRNA-1 or control siRNA, and in HeLa cells transfected with pcDNA3.1-DLEU1 or empty vector pcDNA3.1. (C) The relative luciferase activity in SiHa cells cotransfected with luciferase reporter vectors containing wild-type (WT) DLEU1 or mutant (MUT) DLEU1 and control mimic or miR-381 mimic, and in HeLa cells cotransfected with luciferase reporter vectors containing wild-type DLEU1 or mutated DLEU1 and miR-381 inhibitor or control inhibitor. (D) RIP assay was performed in SiHa cells. DLEU1 and miR-381 expression was detected using qRT-PCR. (E) Cell proliferation (left panel) and invasion (right panel) assessed in SiHa cells transfected with control siRNA + control inhibitor, DLEU1 siRNA-1 + control inhibitor, or DLEU1 siRNA-1 + miR-381 inhibitor. (F) Cell proliferation (left panel) and invasion (right panel) assessed in HeLa cells transfected with control vector + contro1 mimic, DLEU1 vector + contro1 mimic, or DLEU1 vector+ miR-381 mimic. ∗p < 0.05.