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. 2018 Dec 13;1:229. doi: 10.1038/s42003-018-0234-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Identification of GhHB12. a Histochemical localization of GUS activity in proGhHB12:GUS Arabidopsis plants. Scale bars = 2 mm. b Detection of the expression of GhHB12 in stem, leaf, fruit branch bud (FB), vegetative branch bud (VB), and apex of domesticated upland cotton (Gossypium hirsutum L. YZ1) by qRT-PCR. The GhUBQ7 gene was used as the endogenous reference gene. The data represent the mean ± SD of three technical replicates. c–h Photographs of domesticated upland cotton TM-1 (c), YZ1 (f), OE37 (g, GhHB12 overexpression in the YZ1 background), and R19 (h, GhHB12 RNAi in the YZ1 background), ancestral upland cotton morilli (d) and yucatanense (e) plants. Scale bars = 20 cm. i and j Comparison of the number of vegetative branches (i) and the node of the first vegetative branch (NFFB) (j) among YZ1, OE37, and R19 plants. Error bars indicate the standard deviation of 12–15 plants, and different letters indicate significant differences at P < 0.05 (Duncan’s multiple range test). k Physical interaction of GhHB12 with GhSPL10 and GhSPL13 validated using the GAL-4-based Y2H assay. SD synthetic dropout, BD binding domain, AD activation domain, P positive control, N negative control