Table 2.
Effect of diagnosis on oligodendrocytes adjacent to microglia and myelinated fibers
| Oligodendrocytes adjacent to microglia | Oligodendrocytes adjacent to myelinated fibers | |||||||
|---|---|---|---|---|---|---|---|---|
| Controls (n=20) | SCZ (n=21) | F(1,37) | p-value/Cohen’s d | Controls (n=20) | SCZ (n=21) | F(1,37) | p-value/Cohen’s d | |
| Area of oligodendrocyte (μm2) | 61.51±2.6 | 63.57±2.8 | 0.40 | 0.53/0.16 | 63.43±2.4 | 65.05±2.2 | 0.19 | 0.67/0.15 |
| Area of nucleus (μm2) | 18.16±1.0 | 18.44±1.0 | 0.08 | 0.76/0.1 | 20.08±0.7 | 21.04±0.8 | 0.67 | 0.42/0.27 |
| Area of cytoplasm (μm2) | 43.35±2.0 | 45.13±2.1 | 0.51 | 0.48/0.19 | 43.34±1.9 | 44.01±1.6 | 0.04 | 0.83/0.1 |
| Nucleus/cytoplasm ratio | 0.43±0.0 | 0.41±0.0 | 0.26 | 0.61/0.16 | 0.47±0.0 | 0.48±0.0 | 0.31 | 0.58/0.17 |
| Area of mitochondria (μm2) | 0.51±0.0 | 0.5±0.0 | 0.15 | 0.70/0.19 | 0.53±0.0 | 0.55±0.1 | 0.47 | 0.50/0.12 |
| Vv of mitochondria (%) | 4.53±0.5 | 2.73±0.4 | 7.93 | <0.01**/0.83 | 4.21±0.3 | 2.61±0.4 | 9.56 | <0.01**/0.91 |
| N of mitochondria | 3.7±0.3 | 2.43±0.4 | 6.58 | 0.02*/0.76 | 3.42±0.3 | 2.26±0.3 | 6.69 | 0.01*/0.79 |
| Area of vacuoles (μm2) | 1.38±0.3 | 1.47±0.3 | 0.07 | 0.80/0.1 | 1.67±0.3 | 1.3±0.2 | 0.79 | 0.38/0.38 |
| Vv of vacuoles (%) | 0.92±0.2 | 1.87±0.3 | 6.52 | 0.02*/0.73 | 0.77±0.2 | 1.3±0.2 | 4.37 | 0.04*/0.58 |
| N of vacuoles | 0.35±0.1 | 0.76±0.2 | 6.12 | 0.02*/0.70 | 0.22±0.0 | 0.52±0.1 | 7.98 | <0.01**/0.79 |
| Area of lipofuscin granules (μm2) | 1.62±0.3 | 1.64±0.2 | 0.00 | 0.95/0.01 | 1.89±0.4 | 1.83±0.2 | 0.10 | 0.75/0.05 |
| Vv of lipofuscin granules (%) | 0.64±0.2 | 1.91±0.3 | 17.27 | <0.01***/0.99 | 0.77±0.2 | 1.79±0.3 | 10.7 | <0.01**/0.81 |
| N of lipofuscin granules | 0.19±0.0 | 0.56±0.1 | 15.36 | <0.01***/1.01 | 0.19±0.0 | 0.49±0.1 | 9.38 | <0.01**/0.77 |
| Cell density (N/mm2) | 69.04±9.9 | 59.93±6.2 | 0.79 | 0.38/0.24 | 144.2±22.0 | 100.8±13.6 | 2.92 | 0.10/0.51 |
| Peri-neuronal oligodendrocytes | Peri-capillary oligodendrocytes | |||||||
| Controls (n=15) | SCZ (n=16) | F(1,27) | p-value/Cohen’s d | Controls (n=19) | SCZ (n=20) | F(1,35) | p-value/Cohen’s d | |
| Area of oligodendrocyte (μm2) | 57.69±3.2 | 58.16±2.8 | 0.08 | 0.78/0.04 | 55.46±2.9 | 57.53±2.9 | 0.18 | 0.68/0.16 |
| Area of nucleus (μm2) | 18.19±1.2 | 19.07±1.4 | 0.29 | 0.59/0.17 | 16.22±0.8 | 17.25±1.1 | 0.52 | 0.48/0.24 |
| Area of cytoplasm (μm2) | 39.5±2.3 | 39.09±1.5 | 0.00 | 0.95/0.05 | 39.24±2.3 | 40.28±2 | 0.06 | 0.81/0.1 |
| Nucleus/cytoplasm ratio | 0.46±0.0 | 0.48±0.0 | 0.17 | 0.69/0.18 | 0.42±0.0 | 0.43±0.0 | 0.15 | 0.70/0.12 |
| Area of mitochondria (μm2) | 0.59±0.0 | 0.54±0.0 | 0.64 | 0.43/0.37 | 0.55±0.0 | 0.64±0.1 | 2.21 | 0.15/0.43 |
| Vv of mitochondria (%) | 5.3±0.9 | 3.9±0.7 | 1.13 | 0.30/0.45 | 4.07±0.4 | 3.12±0.4 | 2.08 | 0.16/0.48 |
| N of mitochondria | 3.62±0.6 | 2.7±0.4 | 1.08 | 0.31/0.45 | 2.98±0.3 | 2.28±0.4 | 2.09 | 0.16/0.45 |
| Area of vacuoles (μm2) | 1.01±0.5 | 1.21±0.3 | 0.01 | 0.91/0.19 | 2.42±0.7 | 1.02±0.2 | 4.72 | 0.05/0.7 |
| Vv of vacuoles (%) | 0.61±0.1 | 0.81±0.1 | 3.39 | 0.07/0.48 | 0.79±0.3 | 1.32±0.3 | 0.82 | 0.37/0.36 |
| N of vacuoles | 0.30±0.1 | 0.59±0.1 | 3.77 | 0.06/0.49 | 0.14±0.0 | 0.71±0.2 | 4.23 | 0.05/0.72 |
| Area of lipofuscin granules (μm2) | 1.6±0.3 | 1.57±0.2 | 0.01 | 0.92/0.03 | 1.63±0.3 | 1.96±0.3 | 0.19 | 0.67/0.31 |
| Vv of lipofuscin granules (%) | 0.77±0.4 | 1.27±0.3 | 1.08 | 0.31/0.36 | 0.56±0.2 | 1.68±0.5 | 5.04 | 0.03*/0.66 |
| N of lipofuscin granules | 0.18±0.1 | 0.32±0.1 | 2.14 | 0.12/0.49 | 0.12±0.0 | 0.43±0.1 | 5.71 | 0.02*/0.68 |
| Cell density (N/mm2) | 39.8±14.6 | 50.53±13.8 | 0.19 | 0.67/0.17 | 47.46±7.6 | 58.77±16.3 | 0.42 | 0.52/0.19 |
Estimates of the ultrastructural parameters and cell density (means ± S.E.M.) of oligodendrocytes adjacent to microglia, myelinated fibers, neurons, and capillaries in the control and in the SCZ groups and between-group differences (F, p-values, ANCOVA), Cohen’s d
*p < 0.05; **p < 0.01, ***p < 0.001