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. 2018 Dec 13;8:17835. doi: 10.1038/s41598-018-36388-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gross examination (n = 12), Micro Computerized Tomography (CT) Scan (n = 4), and Von Kossa Stain (n = 10) of Metabolic Syndrome Pig Aortic Cusps after 16 Weeks. Normal diet cusp showing no calcification via (A) gross examination, (B) sliced 2D cross-sectional view, (C) 3D volume rendering, and D) no evidence of calcium deposit utilizing Von Kossa. High fat diet cusp showing (E) Deposited calcific nodules observed (red arrow), (F) sliced 2D cross-sectional view showing areas of opacity (yellow box) along the cusp periphery, (G) 3D volume rendering showing opacity, and H) darken areas (denoted with arrows) shows calcium deposits that are specific to the cusp periphery. (I) The percentage area of calcification in the HF diet group statistical significance (P < 0.05).