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. 2018 Dec 13;8:17805. doi: 10.1038/s41598-018-36205-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hemolytic activity of Cyt1A wild type and mutant proteins assayed in Rabbit red blood cells. Positive control showing 100 percent hemolysis was defined after incubation of the same volume of rabbit red blood cells with dechlorinated H₂O. Negative controls were red blood cells incubated with buffer A. These assays were performed three times in triplicate each time. Standard deviations are shown in the figure.