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. 2018 Dec 13;8:17805. doi: 10.1038/s41598-018-36205-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Oligomerization of Cyt1Aa and mutant proteins after activation of solubilized protoxin, with trypsin in the presence of SUV liposomes. Samples were heated 3 min at 65 °C before loading into the SDS-PAGE transferred to PVDF and reveled in western blot assay as described in materials and methods using polyclonal anti-Cyt1A antibody and goat anti-rabbit antibody coupled to horseradish peroxidase.