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. 2018 Dec 13;8:17828. doi: 10.1038/s41598-018-36181-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Experimental design. Ninety mice were randomized into three groups (n = 30), Group 1: Negative control; Group 2: PBS eye drops, 4 times every day; Group 3: 5.0 mg/ml PM_2.5, 4 times every day. (B-D) Evaluation of the body weight (B), eyeball weight (C), and extra-orbital lacrimal gland weight (D) of the mice used in this study. The body weights, eyeball weights, and lacrimal gland weights of the three groups were not significantly different (P > 0.05). Abbreviations: SD, standard deviation; PBS, phosphate-buffered saline; BW, body weight. Each value represents the mean ± the SD, n = 5. These measures were compared with group 1 at the same time points: *P < 0.05 vs. control, ^vP < 0.05 vs. PBS.