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. 2018 Dec 7;9:3009. doi: 10.3389/fmicb.2018.03009

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Copyright © 2018 Peters, Spatharis, Dario, Dwyer, Roca, Kintner, Kanstad-Hanssen, Llewellyn and Praebel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design aiming to test the efficiency of two sequencing methods, namely Illumina MiSeq and IonTorrent, in reflecting the species composition and abundance in different incubations. Each incubation contained four aquaculture related risk agents either in isolation or combined: Lepeophtheirus salmonis (LeSa), Paramoeba perurans (PaPe), algal mix (AlgMix) containing Prymnesium parvum, Pseudonitzschia delicaticima, and P. seriata, and Mix containing all the species together. Each of these incubations was performed at three abundance levels representing triplings of the initial abundance (i.e., 2, 6, and 18). The experiment aimed to control for the effect of background noise in these treatments thus we deployed these using both filtered and unfiltered seawater medium.