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. 2018 Dec 7;9:1802. doi: 10.3389/fpls.2018.01802

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Copyright © 2018 Guan, Yang, Xia, Shi, Yang, Cheng and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The subcellular localization of CaHSL1. (A) CaHSL1 was localized to the plasma membrane and nucleus when transiently overexpressed in leaves of N. benthamiana that were infiltrated with GV3101 cells containing 35S::CaHSL1-YFP using 35S::YFP as control. The Agrobacterium-infiltrated N. benthamina leaves were harvested at 48 hpi, and counterstained by DAPI. Images were taken by confocal microscopy. Control (35S::YFP) showed signal throughout the cell. Bars = 50 μm. (B) The detection of CaHSL1 in the nucleus and cytoplasm by immunoblotting with total (T), nuclear (N), and cytoplasmic (C) proteins isolated from CaHSL1-FLAG or FLAG transiently overexpressing N. benthamina leaves using antibodies to FLAG. α-FLAG, FLAG antibodies.