Fig. 2.

The utilization of cyanate as a nitrogen source in ascomycete yeasts. Yeast strains were cultured in 3 ml chemically defined minimal medium (Linder 2018) containing either 10 mM of the indicated nitrogen source or no nitrogen supplementation (initial OD₆₀₀ 0.005). Samples were incubated in a shaker set at 30 °C, 200 rev/min, and OD₆₀₀ was measured after 6, 12 and 18 days. Growth assays were performed in triplicate with error bars indicating one standard deviation